Experimental Design Analysis
1. Problem: Analyze the randomized block design data to test if washing solutions affect bacterial growth (α = 0.05).
Step 1: State hypotheses.
- Null hypothesis ($H_0$): No difference among solutions.
- Alternative hypothesis ($H_a$): At least one solution differs.
Step 2: Organize data by solutions (treatments) and days (blocks):
- Solutions: 1, 2, 3
- Blocks (days): 1, 2, 3, 4
Data:
Solution 1: 13, 22, 18, 39
Solution 2: 16, 24, 17, 44
Solution 3: 5, 4, 1, 22
Step 3: Calculate totals and means (solutions and blocks), grand total $G$:
- Total per solution:
$T_1 = 13+22+18+39=92$
$T_2 = 16+24+17+44=101$
$T_3 = 5+4+1+22=32$
- Total per block:
$B_1=13+16+5=34$
$B_2=22+24+4=50$
$B_3=18+17+1=36$
$B_4=39+44+22=105$
- Grand total $G=92+101+32=225$
Step 4: Calculate sums of squares:
- Total sum of squares (SST):
$SST = \sum y_{ij}^2 - \frac{G^2}{N}$, with $N=12$.
Calculate $\sum y_{ij}^2 = 13^2+22^2+...+22^2$
- Treatment sum of squares (SS_T):
$SS_T = \sum \frac{T_i^2}{b} - \frac{G^2}{N}$, where $b=4$ (blocks).
- Block sum of squares (SS_B):
$SS_B = \sum \frac{B_j^2}{t} - \frac{G^2}{N}$, where $t=3$ (treatments).
- Residual sum of squares (SS_E):
$SS_E = SST - SS_T - SS_B$
Step 5: Calculate mean squares (MS), degrees of freedom (df).
- $df_T = t - 1 = 2$
- $df_B = b - 1 = 3$
- $df_E = (t-1)(b-1) = 6$
- $MS_T = SS_T / df_T$
- $MS_B = SS_B / df_B$
- $MS_E = SS_E / df_E$
Step 6: Calculate F-statistic for treatments:
$$F = \frac{MS_T}{MS_E}$$
Step 7: Compare with critical $F_{\alpha, df_T, df_E}$ (at 0.05 level).
- If $F > F_{critical}$, reject $H_0$ and conclude differences among solutions.
2. Problem: Analyze Latin square design to test ingredient effects on reaction time (α = 0.05).
Step 1: Identify factors - treatments (ingredients A-E), rows (batches), and columns (days).
Step 2: Compute totals and sums of squares for treatments, rows, and columns.
Step 3: Calculate total sum of squares, treatment sum of squares, row sum of squares, and column sum of squares.
Step 4: Calculate residual sum of squares.
Step 5: Calculate mean squares and degrees of freedom.
Step 6: Calculate F-statistic for treatments and compare with critical values.
Step 7: Conclude whether ingredient affects reaction time.
3. Problem: Analyze Latin square design data for assembly methods on assembly time (α = 0.05).
Repeat analysis as in problem 2 with treatments (assembly methods), rows (operators), and columns (orders).
Calculate sums of squares, mean squares, F-statistics, and conclude.
4. Problem: Analyze Graeco-Latin square design data including workplaces as fourth factor (α = 0.05).
Step 1: Recognize four factors: treatment (assembly methods), rows (order), columns (operator), and Greek letters (workplace).
Step 2: Calculate sums of squares for all four factors and residual.
Step 3: Calculate mean squares and appropriate F-tests for the main treatment effect.
Step 4: Compare F-statistics to critical values at α = 0.05.
Step 5: Conclude if assembly method and workplaces significantly affect assembly time.
Final: Each design controls different sources of variability (blocks, batches, operators, workplace) to isolate treatment effects, confirming statistical differences if $F$ tests exceed critical values at 5% significance level.